Table 1. Yeast strains used in this study.
| Name | Genotype | Figures | Reference |
|---|---|---|---|
| W303-1a | MATa ade2-1 ura3-1 his3-11,15 trp1-1 leu2-3,112 can1-100 | - | (Thomas and Rothstein, 1989) |
| ScKH146 | W303-1A, ade2::ADE2 ATG5-EGFP-kanMX6
ATG17-2×mCherry-hphNT1 |
1A | This study |
| ScKH153 | ScKH146 atg16Δ::natNT2 | 1A | This study |
| ScKH182 | ScKH146 atg21Δ::zeoNT3 | 1A | This study |
| ScKH151 | ScKH146 atg14Δ::natNT2 | 1A | This study |
| ScKH149 | ScKH146 atg12Δ::natNT2 | 1A | This study |
| ScKH162 | ScKH146 atg14Δ::natNT2 atg12Δ::zeoNT3 | 1A | This study |
| ScTK623 | ScKH146 atg21Δ::natNT2 atg12Δ::zeoNT3 | 1A | This study |
| BJ2168 | MATa leu2 trp1 ura3-52 prb1-1122 pep4-3 prc1-407 gal2 | 2A-C, 2E, 2F, 2-S1A, 2-S1C |
(Jones, 1991) |
| MAN169 | BJ2168 ATG5-TEV-3×FLAG-kanMX4 | 2A, 2C, 2E, 2F, 2-S1A, 2-S1C |
This study |
| ScKH10 | MAN169 atg16Δ::natNT2 | 2A, 2E, 2-S1A |
This study |
| ScKH32 | MAN169 atg12Δ::natNT2 | 2A, 3A | This study |
| ScKH96 | MAN169 atg10Δ::natNT2 | 2A | This study |
| ScKH90 | BJ2168 ATG16-TEV-3×FLAG-kanMX4 | 2B | This study |
| ScKH92 | ScKH90 atg5Δ::natNT2 | 2B | This study |
| ScKH93 | ScKH90 atg12Δ::natNT2 | 2B | This study |
| ScKH141 | MAN169 atg1Δ::natNT2 | 2C | This study |
| ScKH99 | MAN169 atg13Δ::natNT2 | 2C, 2D | This study |
| ScKH216 | MAN169 atg17Δ::natNT2 | 2C | This study |
| ScKH101 | MAN169 atg29Δ::natNT2 | 2C | This study |
| ScKH143 | MAN169 atg31Δ::natNT2 | 2C | This study |
| ScKH98 | MAN169 atg14Δ::natNT2 | 2E | This study |
| ScKH97 | MAN169 atg11Δ::natNT2 | 2F | This study |
| ScYH3184 | BJ2168 leu2::LEU2 | 2G | This study |
| ScKH66 | ScHY3184 ATG5-TEV-3×FLAG-kanMX4 | 2G | This study |
| ScKH68 | ScHY3184 atg1D211A-hphNT1 ATG5-TEV-3×FLAG-kanMX4 | 2G | This study |
| AH109 | MATa trp1-901 leu2-3, 112 ura3-52 his3-200 gal4Δ gal80ΔLYS2::GAL1UAS-GAL1TATA-HIS3 MEL1 GAL2UAS-GAL2TATA-ADE2 URA3::MEL1UAS-MEL1TATA-lacZ | 2-S2A | Clontech |
| ScTK967 | AH109 atg13Δ::natNT2 atg31Δ::hphNT1 | 2-S2B | This study |
| ScTK968 | AH109 atg17Δ::natNT2 | 2-S2C | This study |
| ScTK877 | BJ2168 atg11Δ::LEU2 atg29Δ::zeoNT3 atg31Δ::hphNT1 atg1Δ::natNT2 atg13Δ::Klura3 PADH1-ATG17-CgTRP1 | 2-S2D | This study |
| ScTK958 | ScTK877 KlURA3-PADH1-ATG12-EGFP-kanMX4 | 2-S2D | This study |
| ScTK649 | ScTK623 his3-11::pRS303 | 3B | This study |
| ScTK650 | ScTK623 his3-11::pRS303-ATG12 | 3B | This study |
| ScTK651 | ScTK623 his3-11::pRS303-atg12ΔN56 | 3B | This study |
| BY4741 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | - | (Brachmann et al., 1998) |
| ScTK557 | BY4741 pho8Δ::kanMX4- PGPD-pho8Δ60 atg12Δ::natNT2 | 3C, 4A | This study |
| ScTK559 | BY4741 pho8Δ::kanMX4- PGPD-pho8Δ60 atg21Δ::zeoNT3 atg12Δ::natNT2 | 3C, 4A | This study |
| ScKH119 | W303-1A, ade2Δ::ADE2 ATG17-EGFP-kanMX4 atg11Δ::zeoNT3 | 4B | This study |
| ScKH121 | ScKH119 atg5Δ::natNT2 | 4B | This study |
| ScKH123 | ScKH119 atg12Δ::natNT2 | 4B | This study |
| ScKH125 | ScKH119 atg16Δ::natNT2 | 4B | This study |
| ScTK657 | YKH123 his3-11::pRS303 | 4C | This study |
| ScTK658 | YKH123 his3-11::pRS303-ATG12 | 4C | This study |
| ScTK659 | YKH123 his3-11::pRS303-atg12ΔN56 | 4C | This study |