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. Author manuscript; available in PMC: 2019 Mar 11.
Published in final edited form as: Leukemia. 2018 Sep 11;33(3):671–685. doi: 10.1038/s41375-018-0248-0

Figure 3. DNMAML expression in Kras hematopoietic system reduces myeloid compartment.

Figure 3

Lethally irradiated mice (CD45.1+) were transplanted with 2 ×106 splenocytes (CD45.2+) from KrasLSL G12D/+;Mx1-Cre (Kras) or KrasLSL G12D/+;Rosa26LSL DNMAML-GFP/+;Mx1-Cre (Kras; D/+) mice along with 2.5×105 competitor cells (CD45.1+). Three weeks after transplantation, Cre expression was induced using pI-pC injections as described in Methods. Recipients transplanted with Kras or Kras; D/+ cells were sacrificed 3 weeks after pI-pC injections. Donor-derived cells are defined as CD45.2+ cells in Kras recipients and CD45.2+ GFP+ cells in Kras; D/+ recipients. (A) Quantification of donor-derived hematopoietic stem cells (HSCs), multi-potential progenitors (MPPs), myeloid progenitors (MPs), and common lymphoid progenitors (CLPs) in the bone marrow (BM) and spleen (SP) of recipients. (B) Quantification of donor-derived common myeloid progenitors (CMPs), granulocyte-macrophage progenitors (GMPs), and megakaryocyte-erythroid progenitors (MEPs) in the bone marrow of recipients. (C) Quantification of donor-derived differentiated cells in BM, SP, and peripheral blood (PB). Data are presented as mean ± SD. * P<0.05, ** P<0.01; *** P<0.001.