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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: Neurobiol Aging. 2018 Dec 6;75:198–208. doi: 10.1016/j.neurobiolaging.2018.11.023

Fig.2.

Fig.2.

Overexpression of CIP2A induces reactive astrogliosis. Primary rat astrocytes (DIV15) were infected with AAV-CIP2A for 5 days. (A) Immunofluorescence staining of GFAP (red) and Hoechst (blue). Scale bar = 40 μm. (B) The numbers of astrocytes were counted in control and CIP2A overexpression group, n = 9 well per group. (C) Left: CIP2A and GFAP expression levels were detected by Western blotting. DM1A was used as a loading control. Right: Quantitative analysis of the CIP2A and GFAP levels, n = 3 per group. (D) IL-1α, IL-6 and TNF-α in culture media were detected by ELISA, n = 9 well per group. (E) Aβ40 and Aβ42 levels in culture media were detected by ELISA, n = 9 well per group. *p < 0.05, **p < 0.01, ***p < 0.001 versus Vector. Data are mean ± SEM.