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. Author manuscript; available in PMC: 2019 Aug 27.
Published in final edited form as: Nature. 2019 Feb 27;567(7746):127–131. doi: 10.1038/s41586-019-0990-0

Extended Data Figure 4 |. 3D negative stain EM reconstruction of the KCTD12 full-length complex including KCTD12 full length, GABAB2Cter and Gβ1γ2.

Extended Data Figure 4 |

a, Three different views of the three-dimensional reconstruction of full length KCTD12-Gβ1γ2 complex with KCTD16BTB-GABAB2 peptide complex (red) and KCTD12H1- Gβ1γ2 complex (blue-magenta) crystal structures docked into the negative stain electron microscopy envelope. Negative stain 2D class averages are reproduced from Figure 2b for reference. b, The Fourier shell correlation (FSC) curve as a function of spatial frequency for negative staining EM map. Resolution is indicated below. c, Structure of full-length KCTD12 complex. The BTB domain is separated from H1 domain by 35 Å. Dashed lines represent the linker sequence between BTB and H1 domains.