FIGURE 9.

Field emission scanning electron microscopy (FESEM) images of onion cell wall without enzyme treatment (a,a’) and after different enzyme treatments (b–d,b’–d’). (e–g) Effect of treatments on wall properties. XGase is a family-12 glycosyl hydrolase (GH) that hydrolyzes xyloglucan but not unbranched glucan, Case is a family-12 GH that hydrolyzes non-crystalline cellulose only, and CXGase is a family-5 GH that hydrolyzes both non-crystalline cellulose and xyloglucan. No EG denotes no endoglucanase treatment. Reprinted with permission from Zheng et al. (2018). Xyloglucan in the primary cell wall: assessment by FESEM, selective enzyme digestions and nanogold affinity tags. The Plant Journal 93, 211–226. Copyright 2018, John Wiley and Sons.