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. 2019 Mar 1;10:137. doi: 10.3389/fgene.2019.00137

FIGURE 5.

FIGURE 5

Self- and out-cross fertility assays of ATCC42981_R wild type and MATαPΔ deletion mutant. (A) Shows the scheme of self- and out-cross fertility assays involving ATCC42981_R wild type and MATαΔ clone_74, while (B) depicts representative phase contrast microscopic images of control strain Zygosaccharomyces parabailii G21C grown in YPDA and MEA media. (C) Shows selected phase contrast microscopic images of representative ATCC42981_R MATαΔ clone_74 grown as monoculture and in mixture with ATCC42981_R wild type, CBS4837 (α) and CBS4838 (a). Phase contrast microscopic images of ATCC42981_R wild type, CBS4837 and CBS4838 monocultures were reported for comparative purposes. The scale bar represents 11 μm and was reported in only one picture for brevity. Capital letters from A to E indicate abortive shmoo, conjugated asci with ascospores, conjugative bridge, giant cells, and abnormal conjugative tube, respectively. All cells were photographed after 7 days on incubation on MEA medium at 27°C. wt, wild type.