Figure 2.
Number of reads per amplicon length. The left panel shows unambiguous heterozygote genotypes for tri‐,tetra‐,and pentanucleotide motifs. The right panel shows examples matching the three cases of the automatic stutter control: Case I, two alleles with a length difference above the repetition motif length; case II, two alleles with length difference equal to the motif length, whose the frequency of the shorter is higher than the longer one; case III, two alleles with length difference equal to the motif length, whose the frequency of the shortest allele is more than 75% of the longer one. Green bars correspond to amplicon lengths chosen as alleles by the genotyping method. Numbers above each bar indicate the allele length. The line above each graph indicates the chosen genotype and the corresponding number of reads supporting it