Table 1.

Average, across used loci, of amplification success shown as percentage of missing data and average variability measures: N _a—number of alleles, H _O—observed heterozygosity, H _E—expected heterozygosity, and PIC—polymorphism information content. Values in brackets correspond to minimum and maximum values. Values calculated based on sequence information are represented by the superscript “S” while the ones based on length information by “L”. Statistics were calculated based on different markers and samples sets

Statistics	Marker set	All samples	E. europaeus	E. roumanicus
% missing	All	15.48 (0–47.56)	14.75 (0–85.37)	16.2 (0–85.37)
	E. europaeus	15.39 (0–47.56)	18.01 (0–85.37)	12.76 (0–60.98)
	E. roumanicus	15.62 (0–45.12)	9.45 (0–51.22)	21.8 (0–85.37)
$N_{\text{a}}^{\text{L}}$	All	9.98 (2–23)	7.12 (2–17)	7.1 (2–16)
	E. europaeus	8.96 (3–19)	6.38 (2–13)	6.65 (3–13)
	E. roumanicus	11.63 (2–23)	8.31 (2–17)	7.81 (2–16)
$N_{\text{a}}^{\text{S}}$	All	16.83 (4–50)	10.45 (3–28)	10.38 (3–34)
	E. europaeus	15.58 (5–49)	9.54 (3–28)	10.15 (3–25)
	E. roumanicus	18.88 (4–50)	11.94 (3–23)	10.75 (4–34)
$H_{\text{o}}^{\text{L}}$	All	0.45 (0.09–1)	0.44 (0–1)	0.46 (0–1)
	E. europaeus	0.39 (0.09–0.97)	0.35 (0–0.97)	0.43 (0–0.98)
	E. roumanicus	0.55 (0.12–1)	0.59 (0.1–1)	0.51 (0.1–1)
$H_{\text{o}}^{\text{S}}$	All	0.52 (0.12–1)	0.51 (0.03–1)	0.51 (0–1)
	E. europaeus	0.47 (0.12–0.99)	0.44 (0.03–0.97)	0.49 (0–1)
	E. roumanicus	0.59 (0.12–1)	0.61 (0.1–1)	0.55 (0.12–1)
$H_{\text{E}}^{\text{L}}$	All	0.74 (0.25–0.94)	0.6 (0.05–0.89)	0.64 (0.09–0.92)
	E. europaeus	0.72 (0.47–0.92)	0.51 (0.05–0.89)	0.64 (0.09–0.9)
	E. roumanicus	0.76 (0.25–0.94)	0.74 (0.31–0.89)	0.65 (0.13–0.92)
$H_{\text{E}}^{\text{S}}$	All	0.81 (0.49–0.97)	0.68 (0.07–0.94)	0.71 (0.11–0.96)
	E. europaeus	0.8 (0.57–0.95)	0.62 (0.07–0.94)	0.71 (0.11–0.94)
	E. roumanicus	0.83 (0.49–0.97)	0.78 (0.41–0.94)	0.71 (0.16–0.96)
PICL	All	0.7 (0.23–0.93)	0.56 (0.05–0.87)	0.6 (0.09–0.91)
	E. europaeus	0.68 (0.37–0.91)	0.48 (0.05–0.87)	0.59 (0.09–0.86)
	E. roumanicus	0.74 (0.23–0.93)	0.7 (0.29–0.87)	0.61 (0.12–0.91)
PICS	All	0.78 (0.46–0.96)	0.67 (0.11–0.94)	0.64 (0.07–0.93)
	E. europaeus	0.77 (0.48–0.94)	0.67 (0.11–0.9)	0.58 (0.07–0.93)
	E. roumanicus	0.81 (0.46–0.96)	0.67 (0.16–0.94)	0.74 (0.38–0.92)