Figure 3.
Platelet NF‐κB activation by sCD40L is CD40‐dependent. Washed human platelets (1000×106/mL) were treated with (A) 10 μg/mL of ReoPro, (B) 10 μg/mL of JBS5, or (C) 10 μg/mL of 5C8 and corresponding immunoglobulin Gs for 5 minutes at 37°C. Pretreated platelets were then stimulated with 1000 ng/mL of sCD40L for 5 minutes. Platelet lysates were resolved in 10% SDS–PAGE and assessed for pIκBα and pP65. Actin blot is from stripped membranes of pIκBα blot; and P65 blot is from stripped membranes of pP65 blot. Blots are representative of 4 independent experiments. Histograms represent the mean of data, expressed in optical density (n=4, mean±SEM). IgG indicates immunoglobulin G. *P<0.001 (one‐way ANOVA followed by Dunnett's multiple comparisons test vs the sCD40L group).