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. 2019 Feb 16;8(4):e010866. doi: 10.1161/JAHA.118.010866

Figure 3.

Figure 3

Intermittent fasting (IF) promotes myocardial protein aggregate removal in αB‐crystallin R120G mutant transgenic mice. A through C, Representative immunoblot (A) with quantitation of αB‐crystallin in the soluble (B) and insoluble (C) myocardial fractions from Myh6‐CryABR120G transgenic mice subjected to IF or ad‐lib (AL) feeding, and littermate wild‐type (WT) controls at 46 weeks of age. N=4/group. P value is by t test. “Darker” and “lighter” indicate relative exposures of the same blot. D, Representative TEM (Transmission electron microscopy) images from Myh6‐CryABR120G transgenic mice subjected to IF or AL feeding and littermate WT mice. White arrows point to protein aggregates. Black arrows point to autolysosomes. Black arrowheads point to Z‐discs, where intracellular desmin is localized, pointing to partial restoration of Z‐disc architecture in intermittently fasted Myh6‐CryABR120G transgenic mice compared with AL fed controls. Representative of N=2/group.