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. 2018 Dec 21;8(1):e010332. doi: 10.1161/JAHA.118.010332

Figure 5.

Figure 5

Mechanical tension and exosome secretion of miR‐133a. Exosomes were precipitated from conditioned culture media from each fibroblast and smooth muscle cell (SMC) line. A, Acetylcholinesterase activity was quantitated as a measure of exosome number from each fibroblast line exposed to 12% biaxial cyclic stretch (Tension, n=8) or held static (Control, n=8) for 18 hours. B, miR‐133a abundance in exosomes precipitated from the conditioned media of each fibroblast line exposed to 12% biaxial cyclic stretch (Tension, n=7) or held static (Control, n=7) for 18 hours. C, miR‐133a abundance in each aortic fibroblast line after exposure to 3 hours of 12% biaxial cyclic stretch in the presence of 20 μmol/L GW4869 (Tension+GW4869, n=8) or held static (Control, n=8). D, Acetylcholinesterase activity was quantitated as a measure of exosome number from each SMC line exposed to 12% biaxial cyclic stretch (Tension, n=6) or held static (Control, n=6) for 18 hours. E, miR‐133a abundance in exosomes precipitated from the conditioned media of each SMC line exposed to 12% biaxial cyclic stretch (Tension, n=6) or held static (Control, n=6) for 18 hours. A through E, Data are represented in dot plots with the mean and SEM shown next to each group. Comparisons were made using a 2‐sample t test (unpaired, 2 tailed). *P<0.05 vs control.