Fig. 5.

Vaccine-driven virus resolution is CD4⁺ and CD8⁺ cell-dependent. Lewis rats were injected intramuscularly with 5 × 10⁸ ifu Ad-NSmut. After 3 weeks, rats were challenged intravenously with 10⁶ genomes RHV. a–c At days −2, 5, and 12 post infection, rats were transiently depleted of CD8α⁺ cells by antibody or isotype control. a Number of CD8⁺ T cells/mL blood of IgG1 and anti-CD8α antibody-treated rats as determined by flow cytometric analysis. Arrows indicate timing of antibody administration. Data from n = 4 rats per group are shown (mean ± SEM). b, c Viremia in IgG1 and anti-CD8α antibody treated rats. Solid line with below shading indicates limit of quantification (LOQ) of RT-PCR. d–f At days −3, −1, and 4 post infection, rats were transiently depleted of CD4⁺ cells by antibody or isotype control. d Number of CD4⁺ T cells/mL blood of IgG2a and anti-CD4 antibody-treated rats as determined by flow cytometric analysis. Arrows indicate timing of antibody administration. Data from n = 4 rats per group are shown (mean ± SEM). e, f Viremia in IgG2a and anti-CD4 antibody treated rats. Solid line with below shading indicates LOQ of RT-PCR