Table 1.
Characteristics of DMD patients.
| Subjects | Age (years) | Sample types | DMD exon deletion | Target exon for skipping | Forward primer (5′ to 3′) | Reverse primer (5′ to 3′) |
|---|---|---|---|---|---|---|
| DMD-1 | 6 | Skin & Urine | 45–54 | 44 | ATGCTCCTGACCTCTGTGCT | GACTGCATCATCGGAACCTT |
| DMD-2 | 11 | Skin & Urine | 45 | 44 | GCTCAGGTCGGATTGACATT | GGGCAACTCTTCCACCAGTA |
| DMD-3 | 5 | Urine | 50 | 51 | ACAACCGGATGTGGAAGAGA | GCCTCTTGATTGCTGGTCTT |
| DMD-4 | 13 | Urine | 51 | 50 | ACAACCGGATGTGGAAGAGA | GCCTCTTGATTGCTGGTCTT |
| DMD-5 | 10 | Urine | 54 | 55 | CGGGCTTGGACAGAACTTAC | GACTGCATCATCGGAACCTT |
DMD exon deletions were restored in-frame by skipping the target exon. Primers were used for RT-PCR to evaluate exon-skipping efficiency. DMD: Duchenne muscular dystrophy.