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. 2019 Mar 7;9:3824. doi: 10.1038/s41598-019-40569-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of NO₃⁻ availability at vegetative stage. Plants were grown on sand for 35 days, watered with nutritive solution containing 0.1, 2 or 10 mM NO₃⁻. (a) Fresh weight of total plant, roots and shoots. (b) Schematics of tiller development. Tiller were numbered according to their sequential apparition. (c) Fresh weight, (d) N content, (e) Free AA content and (f) NO₃⁻ content of main stem and tillers. Values correspond to the mean of 4 or 5 biological replicates (2 plants each) +/−SD. Letters indicate statistical groups for each plant part (Non-parametric ANOVA, p < 0.05). N/a: no data available, due to the lack of development of tillers 2 and 3 in the 0.1 mM NO₃⁻ condition.