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. 2019 Mar 7;9:3824. doi: 10.1038/s41598-019-40569-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of pre- and post-anthesis NO₃⁻ availability on grain composition. Growing conditions and treatments were as described in Fig. 2. LN, Ctrl and HN: 0.1, 1 and 10 mM NO₃⁻, respectively. Grain contents in (a) total N (GNC), (b) extractable proteins and (c) free AA. The boxplots represent minimum, 1^st quartile, median, 3^rd quartile and maximum values. Letters indicate statistical groups (Non-parametric ANOVA, p < 0.05). Number of biological replicates for N content are indicated above the chart; other charts are based on 5 biological replicates per condition.