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. 2019 Mar 7;9:3824. doi: 10.1038/s41598-019-40569-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of pre- and post-anthesis NO₃⁻ availability on grain free AA composition. Growing conditions and treatments were as described in Fig. 2. LN, Ctrl and HN: 0.1, 1 and 10 mM NO₃⁻, respectively. Only free AA levels affected by the treatments are presented; see Table S1 (Supplementary Information) for full set of data. Values are means of 5 biological replicates +/−SD. Letters indicate statistical groups for each AA (Non-parametric ANOVA, p < 0.05).