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. 2019 Mar 7;9:3824. doi: 10.1038/s41598-019-40569-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of post-anthesis NO₃⁻ availability on N fluxes. Plants were grown hydroponically on 0.2 mM NO₃⁻ and transferred at anthesis to (a) 0.01, (b) 0.2 or (c) 10 mM NO₃⁻. Left arrows (red): post-anthesis remobilization of pre-anthesis N pools from vegetative plant parts. Right arrows (blue): post-anthesis NO₃⁻ uptake. Arrows indicate the N fluxes from/to spikes, shoots or roots; values of the fluxes (mg_N) are specified above the arrows. Bold values at the bottom of the panels correspond to total N remobilization (left) and total NO₃⁻ uptake (right) at the plant level (mg_N). Percentages above the spikes indicate the proportion of grain N originating from remobilization (left) and from post-anthesis uptake (right). Total N quantities in spikes, shoots and roots after full senescence of the plants are indicated by circles on the right of each panel; absolute values (mg_N) are indicated; distribution of N quantity between spikes, shoots and roots are specified in brackets (% of the plant total N). Values correspond to the mean of 5 (panel a) or 7 (panels b and c) biological replicates +/−SD. Raw data (DW, N content and ¹⁵N enrichment of the different plant parts) and results of statistical tests are presented in Supplementary tables ST2 and ST3, respectively.