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. 2019 Mar 7;9:3824. doi: 10.1038/s41598-019-40569-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Response of BdNRT2/3 expression to N availability at vegetative stage. Plants were grown in hydroponics for 17 days under the indicated N condition. (a) Response of BdNRT2 genes to NO₃⁻ availability. BdNRT2A and BdNRT2B were quantified simultaneously due to high sequence homology. ND: not detected. (b) Response of BdNRT2A/B expression to NH₄⁺. (c) Response of BdNRT3 genes to NO₃⁻ availability. Values are normalized by BdEF1α expression; similar results were obtained after normalization by BdUBC18 or BdUbi10. Values correspond to the mean of 4–5 biological replicates +/−SD. Letters indicate statistical groups for each gene and plant organ system (ANOVA on Log2-transformed values, p < 0.05).