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. 2019 Mar 7;9:3793. doi: 10.1038/s41598-019-40507-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Retinal structure and function in obe^td15 zebrafish. (a) Wholemount morphology of adult wild-type (WT) and obe^td15 zebrafish. (b) Retinal histology of obe^td15 zebrafish at 3, 6 and 12 months post fertilization (mpf). (c) Expression of kcnj13 mRNA (green) in the WT adult zebrafish retina detected using an RNAscope assay. Sections are counterstained with DAPI nucleic acid stain (blue). (d) Visual acuity (cycles per degree) of obe^td15 zebrafish at 3, 6 and 12 mpf, measured using optokinetic response assay (minimum n = 4, mean ± SEM). (e) Retinal thickness (µm) of obe^td15 zebrafish at 3, 6 and 12 mpf, measured using OCT (n = 5 for each age, mean ± SEM). GCL, ganglion cell layer; IPL, inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer; IS/OS, outer and inner segments; RPE, retinal pigment epithelium. *p < 0.05, **p < 0.01. Scale bars = 50 μm (b) and 25 μm (c).