. 2019 Mar 7;9:3843. doi: 10.1038/s41598-019-39872-1

Table 1.

Kinetic properties and inhibitory activities in [³H]DA uptake in WT and mutated hDAT.

	V_max (pmol/min/10⁵ cells)	K_m (µM)	IC₅₀ (nM)
	V_max (pmol/min/10⁵ cells)	K_m (µM)	DA	Cocaine	GBR12909	AMPH
WT hDAT	12.52 ± 0.68	1.21 ± 0.17	2063 ± 582.5	350 ± 49.4	511 ± 56.6	926 ± 167.4
Y88F/H547A	24.06 ± 4.32*	4.25 ± 1.05*	7918 ± 637.6*	131 ± 13.9*	253 ± 40.4*	1279 ± 200.5
Y88F/ K92M/H547A	0.5 ± 0.31*	1.89 ± 1.25	ND	ND	ND	ND

Data are presented as mean ± S.E.M. values from four to six independent experiments performed in duplicates. *p < 0.05 compared with WT hDAT (unpaired Student’s t test). ND. Not determined.