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Study design. Estrous cycle stages were assessed daily for a minimum of 2 weeks prior to ozone exposure experiments. Female mice at different stages of the estrous cycle were exposed to 2 ppm of ozone or filtered air (FA, control) for 3 h between 11:00 am and 2:00 pm (n = 6–8 per phase/exposure group). Four hours after exposure (6:00 pm), blood and lung tissue samples were harvested. Lung function testing and BALF collection were performed at 24 h postexposure (11:00 am).
