Table 1.

Characteristics of the newly developed microsatellite markers

Locus name	Motif	Forward primer	Reverse primer	Fluorescent	Size range	Number of alleles
						O. crassus	O. kawanabei	O. assimilis
Olam01	AC	GTCGAACTCCTGCAACTGCT	GGCCTAATTCTTTCCCTGCT	FAM	112–126	1	6	7
Olam02	AG	ACCAGATGTTGGCATGATGA	AGTCAAGGACGGTGCAATTT	VIC	165–187	8	NA	NA
Olam11	AC	GTGATTTAAAGCGCTCTCCG	GTTTCTTCATCGAACCACGAACAAATG	NED	258–266	3	NA	NA
Olam13	AG	CATCATCCAGAGCAAACGAA	GTTTCTTCTTGCAGTCAGTTTGGGTGT	VIC	223–239	6	NA	NA
Olam14	AC	CCCGGTTGAAGATAATTCCA	GTTTCTTCGTGAGGACGCTTCACTGT	NED	132–140	4	1	NA
Olam16	AG	GTGACGGGTAGGAAACTTGC	GCTTTAAAGTTAATGTACCTGCTTTC	VIC	187–191	3	NA	NA
Olam17	AG	ATTTGTTATCGCTATCGGCG	GTTTCTTCGTTTGTCACTGACCGGAA	PET	218–236	5	8	10
Olam18	AG	CAAGAGCAGGCGTAAGTAAATTAAA	GTTTCTTTCCGTAATCCCAGACGACAT	FAM	158–190	6	12	6
Olam19	AG	ACACATTCGAGCAGATTTCG	GTTTCTTGTGCAGTTAGACCGCTCGTT	VIC	251–289	6	3	15
Olam27	AAGAG	TACACGAGACAGTGTGCCCT	TGTCAGAATCGCTCTTGTGG	NED	116–191	NA	11	NA
Olam31	AAT	GCGACTCTCGTTGCTAGGTG	GCGTCACCGTTTCACAATC	PET	182–188	NA	3	NA
Olam32	AAT	TCTCGTCTGCAACATTCTTCA	CGAAACGTAGAGAAGAGTAACCG	VIC	172–184	1	5	NA
Olam35	AAT	CATCTGTTTGATGATCAGTCCC	TACTCAAGCAGTGCACCCAG	NED	129–150	NA	8	NA
Olam39	AAT	GGGTGAAATTTGAGCACGA	TTTCCTATGGCCCAGTTTACA	PET	150–168	NA	NA	7
Olam43	AAT	TGAGCAACTACATTGCGACA	GCATCTAGGGTTGGAACGAC	PET	168–177	1	4	NA
Olam51	AG	TGCACCAACCAGTTATGCTG	GTTTCTTACATTATTCTCACAGAACGTCTTCG	FAM	158–184	NA	NA	12
Olam54	AG	TGTGAACGGGTTGAAATCTG	CGAGAGCGTCGCAAATCTAT	PET	222–260	1	1	16
Olam55	AC	CATTTCCAGAGCGTAATCAAG	CTATACATTCGGCGCGATTC	NED	254–282	NA	NA	13
Olam59	AG	TATGGCTTCCCATATCCTCG	CTAATGGATGCTCGCGATTC	VIC	253–309	NA	8	15
Olam60	AG	CTAAGAGGCGATATGTATTTCGAAGG	AAGAACAATCGAATCACGCC	VIC	199–207	NA	NA	5
Olam65	ACG	CAGTGAACCGGATGTGTACG	TCACCTTCGGTCTGTGTTTG	NED	206–236	6	NA	NA

NA indicates that the locus was not amplified.