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. 2019 Mar 7;9:3788. doi: 10.1038/s41598-019-39789-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Bioenergetics of the Hs578T and Hs578Ts(i)₈ cell variants. (a,b) There is a significant decrease in the rate of basal oxygen consumption (OCR) in the Hs578Ts(i)₈ cells compared to the Hs578T cells. Addition of oligomycin A resulted in a significant decrease in the OCR of the Hs578Ts(i)₈ cells and thus a significant difference in the OCR between the Hs578Ts(i)₈ and Hs578T variants. Following addition of FCCP, OCR significantly increased in the Hs578Ts(i)₈ cells. Finally, upon addition of rotenone, oxygen consumption was decreased substantially, a significant decrease from Hs578Ts(i)₈ cells’ maximal OCR. (c,d) A significant increase in the basal rate of glycolysis was observed in the Hs578Ts(i)₈ cells compared to the Hs578T cells. Following oligomycin A addition, the ECAR increased significantly in the Hs578Ts(i)₈ cells compared to the Hs578T cells. Finally, following addition of 2-DG the ECAR decreased substantially in the Hs578Ts(i)₈ and Hs578T cells. (e) The ratio of the basal and maximal OCR and ECAR is shown, this indicates that the OCR/ECAR ratios were significantly decreased in the Hs578Ts(i)₈ cells compared to the parental Hs578T cells. Data is expressed as the mean ± SEM of n = 3 experiments, where **/^##p < 0.01, ***/^###p < 0.001, *Hs578Ts(i)₈ vs Hs578T vs, ^#Hs578T vs Hs578T.