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. 2019 Mar 7;9:3788. doi: 10.1038/s41598-019-39789-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Evaluation of potential alterations in the mitochondrial biomass. Analysis of mitochondrial biomass by (a). The citrate synthase assay and (b). Immunoblot for VDAC1 showed no significant differences between Hs578T and Hs578Ts(i)₈ cell variants. Data is expressed as the mean ± SEM of n = 3 experiments, differences were not statistically significant (n.s.). Note: the immunoblot was cropped to approximately 6 gel bandwidths of the relevant band. VDAC1 was probed for, the transmembrane was stripped and re-probed with the anti-β-actin antibody. Due to the large qualities of VDAC1 in the cell lysates, complete stripping did not occur and faint VDAC1 bands are still visible when presenting β-actin, due to the large quantities of VDAC1. However, their size differences make them clearly distinguishable.