Table 2.
Relative composition of CSF proteins bound to each fibril system.
| CSF | Ab1 | Ab2 | Glucagon | C-36 | αSN | FAS4-AD | ||
|---|---|---|---|---|---|---|---|---|
| Total protein ion count | 1.2 ·106 | 4.4 ·104 | 2.6 ·104 | 2.6 ·105 | 6.5 ·104 | 5.0 ·103 | 3.5 ·103 | |
| Apolipoproteins (% of total protein) | 1.7 ± 0.1 | 26.3 ± 2.7 | 21.9 ± 3.1 | 11.2 ± 3.4 | 30.2 ± 8.0 | 30.8 ± 7.5 | 36.2 ± 12.8 | |
| P02647 | Apolipoprotein A-I# | 0.4 ± 0.1 | 3.9 ± 0.4 | 2.9 ± 0.6 | 2.6 ± 0.8 | 5.0 ± 1.6 | 2.7 ± 1.0 | * |
| P02652 | Apolipoprotein A-II# | 0.0 ± 0.0 | 1.0 ± 0.3 | * | 0.6 ± 0.2 | 0.9 ± 0.1 | — | — |
| P06727 | Apolipoprotein A-IV | 0.0 ± 0.0 | 1.2 ± 0.1 | 0.5 ± 0.0 | 0.5 ± 0.1 | 1.0 ± 0.1 | 1.5 ± 0.2 | — |
| P05090 | Apolipoprotein D# | 0.3 ± 0.0 | 8.7 ± 0.4 | 11.2 ± 0.6 | 1.8 ± 0.3 | 9.0 ± 2.3 | 3.4 ± 0.5 | 5.7 ± 0.4 |
| P02649 | Apolipoprotein E# | 0.5 ± 0.0 | 2.2 ± 0.5 | 0.4 ± 0.2 | 3.2 ± 1.5 | 5.6 ± 2.1 | 11.0 ± 3.0 | 7.6 ± 1.3 |
| P10909 | Clusterin# | 0.5 ± 0.0 | 9.4 ± 1.0 | 6.9 ± 1.6 | 2.4 ± 0.6 | 8.6 ± 1.7 | 12.2 ± 2.9 | 22.8 ± 11.2 |
| Immunoglobulins (% of total protein) | 23.1 ± 1.4 | 6.7 ± 0.3 | 8.1 ± 0.8 | 4.5 ± 1.3 | 2.9 ± 0.6 | 4.2 ± 0.4 | 0.0 ± 0.0 | |
| P01876 | Ig α-1 chain C region | 1.9 ± 0.1 | * | * | 0.2 ± 0.1 | 0.5 ± 0.1 | — | — |
| P01857 | Ig γ-1 chain C region | 8.5 ± 0.2 | 2.5 ± 0.1 | 3.1 ± 0.3 | 1.4 ± 0.5 | 1.4 ± 0.2 | 4.2 ± 0.4 | * |
| P01859 | Ig γ-2 chain C region | 3.9 ± 0.1 | 1.7 ± 0.2 | 2.0 ± 0.3 | 1.1 ± 0.3 | 1.0 ± 0.3 | * | |
| P01860 | Ig γ-3 chain C region | 7.8 ± 0.3 | 2.5 ± 0.1 | 3.0 ± 0.2 | 1.4 ± 0.5 | * | * | — |
| P01834 | Ig κ chain C region | 1.0 ± 0.7 | * | * | 0.5 ± 0.0 | * | * | * |
| Blood coagulation (% of total protein) | 0.7 ± 0.0 | 4.1 ± 0.5 | 3.8 ± 0.7 | 1.8 ± 0.3 | 2.2 ± 0.4 | 0.0 ± 0.0 | 0.0 ± 0.0 | |
| P01009 | α1-antitrypsin | 0.7 ± 0.0 | 0.5 ± 0.2 | * | 1.0 ± 0.2 | * | * | * |
| P02671 | Fibrinogen α chain | * | 0.4 ± 0.0 | 0.4 ± 0.0 | 0.1 ± 0.0 | * | * | — |
| P02751 | Fibronectin# | * | 0.9 ± 0.0 | 1.2 ± 0.3 | 0.2 ± 0.1 | 0.7 ± 0.1 | * | — |
| P23142 | Fibulin-1 | * | 1.4 ± 0.1 | 1.3 ± 0.1 | 0.3 ± 0.0 | 1.2 ± 0.2 | — | — |
| P04196 | Histidine-rich glycoprotein | * | 0.4 ± 0.1 | 0.6 ± 0.2 | 0.1 ± 0.0 | * | — | — |
| P00734 | Prothrombin | * | 0.4 ± 0.1 | 0.3 ± 0.0 | 0.2 ± 0.0 | 0.4 ± 0.2 | * | — |
| Complement system (% of total protein) | 0.1 ± 0.0 | 1.6 ± 0.3 | 0.9 | 0.1 | 0.9 ± 0.2 | 2.2 ± 0.6 | 0.0 ± 0.0 | |
| P09871 | Complement C1s subcomponent | * | 0.2 ± 0.0 | * | 0.2 ± 0.0 | 0.7 ± 0.2 | — | — |
| P01024 | Complement C3 | 0.1 ± 0.0 | 0.3 ± 0.1 | 0.3 ± 0.1 | 0.8 ± 0.2 | * | — | — |
| P0C0L4 | Complement C4-A | * | 0.3 ± 0.1 | * | 0.3 ± 0.0 | 0.6 ± 0.2 | * | — |
| P08603 | Complement factor H | 0.0 ± 0.0 | 0.8 ± 0.1 | 0.9 ± 0.1 | 0.1 ± 0.0 | * | — | — |
| CSF specific proteins (% of total protein) | 4.7 ± 0.3 | 8.9 ± 0.8 | 12.3 ± 2.1 | 2.0 ± 0.3 | 3.9 ± 0.2 | 7.1 ± 1.4 | 0.0 ± 0.0 | |
| P51693 | Amyloid-like protein 1 | * | 2.8 ± 0.2 | 3.4 ± 0.7 | 0.6 ± 0.0 | 2.0 ± 0.1 | * | — |
| O94985 | Calsyntenin-1# | * | 1.2 ± ± 0.0 | 1.8 ± 0.8 | 0.4 ± 0.2 | 1.2 ± 0.1 | 3.1 ± 0.4 | — |
| P01034 | Cystatin-C | 4.6 ± 0.3 | 3.9 ± 0.4 | 6.0 ± 0.6 | 0.9 ± 0.0 | * | * | * |
| Q9UHG2 | ProSAAS | 0.1 ± 0.0 | 1.0 ± 0.1 | 1.1 ± 0.1 | 0.2 ± 0.0 | 0.7 ± 0.0 | 4.0 ± 0.9 | — |
| Other proteins (% of total protein) | 24.5 ± 1.7 | 46.1 ± 2.3 | 54.1 ± 14.4 | 75.6 ± 2.8 | 54.8 ± 7.5 | 75.1 ± 13.1 | 68.0 ± 14.6 | |
| P02765 | α2-HS-glycoprotein | 0.2 ± 0.0 | 1.1 ± 0.0 | 1.9 ± 0.6 | 0.7 ± 0.2 | 3.6 ± 0.7 | * | — |
| Q9UBP4 | Dickkopf-related protein 3 | 0.3 ± 0.0 | 7.2 ± 0.3 | 5.5 ± 2.2 | 1.5 ± 0.4 | 3.9 ± 1.5 | 10.9 ± 1.0 | — |
| Q12805 | EGF-containing fibulin-like extracellular matrix protein 1 | * | 0.6 ± 0.2 | 1.0 ± 0.3 | 0.2 ± 0.1 | 0.7 ± 0.1 | * | — |
| P06396 | Gelsolin# | 0.4 ± 0.0 | 2.9 ± 0.1 | 3.5 ± 0.6 | 0.8 ± 0.1 | 1.9 ± 0.2 | 5.0 ± 0.9 | — |
| P20774 | Mimecan | * | 0.5 ± 0.0 | 0.6 ± 0.2 | 0.2 ± 0.1 | 0.4 ± 0.1 | 2.1 ± 0.9 | * |
| P02768 | Serum albumin# | 23.6 ± 1.7 | 27.8 ± 1.0 | 33.8 ± 8.8 | 69.7 ± 1.6 | 38.8 ± 3.5 | 49.3 ± 9.1 | 63.6 ± 14.3 |
| Q14515 | SPARC-like protein 1 | * | 1.2 ± 0.1 | 1.4 ± 0.0 | 0.4 ± 0.1 | 0.8 ± 0.4 | * | — |
| P02766 | Transthyretin# | * | 3.0 ± 0.5 | 4.0 ± 1.3 | 1.1 ± 0.2 | 2.4 ± 0.2 | 4.3 ± 1.1 | 4.4 ± 0.3 |
| P02774 | Vitamin D-binding protein | 0.0 ± 0.0 | 0.7 ± 0.0 | 1.1 ± 0.0 | 0.8 ± 0.1 | 1.4 ± 0.8 | — | — |
| P04004 | Vitronectin | * | 1.1 ± 0.0 | 1.3 ± 0.3 | 0.2 ± 0.1 | 0.8 ± 0.1 | 3.6 ± 0.2 | — |
Proteins constituting > 0.5% of the total composition, for at least one fibril system are displayed. Respective fibril peptides have been removed from the quantification. The data represents nine technical replicas with standard deviations.
*Identified, but not quantified. —Not identified in any of the samples. #Significant band in gel analysis.