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. 2019 Feb 1;9(2):270–284.

Figure 5.

Figure 5

DANCR interacts with miR-138. A. Schematic representation of the miR-138 site in DANCR. B. Luciferase activity in A549 cells cotransfected with miR-NC or miR-138 and luciferase reporters containing DANCR or mutant DANCR (DANCR-mut). Data are presented as the relative ratio of firefly luciferase activity to renilla luciferase activity. C. A construct containing DANCR transcripts combined with MS2 binding site (MS2bs) elements. D. MS2-RIP followed by miR-138 qRT-PCR to examine miR-138 endogenously associated with DANCR or mutant DANCR. E. The DANCR expression in A549 cells transfected with miR-NC or miR-138. F. AGO2-RIP followed by DANCR qRT-PCR to assay DANCR level after miR-138 overexpression. G. The miR-138 expression in H1299 cells with overexpression of DANCR or mutant DANCR. H. The miR-138 expression in A549 cells with or without DANCR knockdown. Date are presented as the means ± SD. *P<0.05.