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. 2019 Feb 1;9(2):270–284.

Figure 6.

Figure 6

DANCR upregulates Sox4 expression by competitively binding miR-138. A. The H1299 cells were cotranfected with DANCR and miR-138, and the Sox4 mRNA expression was then detected by qRT-PCR. B. The H1299 cells were cotranfected with DANCR and miR-138, and the Sox4 protein expression was then detected by western blot. C. The A549 cells were cotranfected with DANCR shRNA and miR-138 inhibitor, and the Sox4 mRNA expression was then detected by qRT-PCR. D. The A549 cells were cotranfected with DANCR shRNA and miR-138 inhibitor, and the Sox4 protein expression was then detected by western blot. E. Luciferase activity of Sox4-3’UTR in H1299 cells cotransfected with DANCR and miR-138. Data are presented as the relative ratio of firefly luciferase activity to Renilla luciferase activity. F. Luciferase activity of Sox4-3’UTR in A549 cells cotransfected with DANCR shRNA and miR-138 inhibitor. Data are presented as the relative ratio of firefly luciferase activity to Renilla luciferase activity. Date are presented as the means ± SD. *P<0.05.