Figure 4.

Upregulation of IQGAP3 facilitates phosphorylation of PKCα and activates the AKT signaling pathway. (A) HepG2 cells were transfected with 2 μg pCMV-E2F1, pCMV-IQGAP3 or pCMV vectors. MHCC-97H cells were transfected with E2F1, IQGAP3-specific siRNAs or control siRNAs. Samples were harvested 48 hours after transfection. The levels of E2F1, IQGAP3 and factors in the AKT pathway were determined by immunoblot. (B) Relative expression levels of the proteins indicated in (A). Data are mean ± SEM. Two-tailed Student’s t test was used for statistical calculation.