Figure 6.

Down-regulation of SFRP4 decreased chemokine secretion. A. The mRNA expression of SFRP4 in cell lines. B. The down-regulation of SFRP4 by small interference RNA. C. The expression of cytokines at mRNA level influenced by SFRP4 siRNA. D. The proportion of counting beads and total migrated cell by flow cytometry. E. The gating strategy for the CD4 positive T cells. F. The precision number of total migrated cells and migrated CD4 positive cells. G. The level of β-catenin in the cell plasma and in the nuclear determined by western blot.