Figure 3.
Molecular (a–c) and biological (d–f) changes induced by Echinococcus granulosus Calmodulin (EgCaM)-specific siRNA using three delivery methods in different developmental stages in vitro. (a–c) EgCaM expression profile in different in vitro stages: protoscoleces (PSC) 3 and 8 days after electroporation (EP), soaking (SK) and electro-soaking (ES); microcysts (MC) and strobilated worms (SW). (d) Viability changes of protoscoleces treated with EgCaM-specific siRNA. (e) Size changes of 30 protoscoleces treated with EgCaM-specific siRNA. (f) Changes in body contractions per minute in the strobilated worms treated with EgCaM-specific siRNA. Data was compared to the controls: control protoscoleces (Ctrl) and negative siRNA control (siR-Ctrl). The gene expression data for siR-Ctrl were only demonstrated for electro-soaking method. Bars show the mean ± standard deviation (SD) derived from duplicates experiments. (**P < 0.01, ***P < 0.001, ****P < 0.0001).