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. 2019 Mar 7;9:3894. doi: 10.1038/s41598-019-40656-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Morphological changes in Echinococcus granulosus treated with Calmodulin (EgCaM)-specific siRNA in vitro. Effect of EgCaM suppression on microcysts (a) and strobilated worms (b) using three delivery methods, electroporation (EP), soaking (SK), electro-soaking (ES) compared to the negative siRNA control (siR-Ctrl). Note the morphological changes (arrowheads) in the microcysts (outer layer irregularities and shrinkage, malformations and darkened edges) and the strobilated worms (morphological abnormalities and body swelling) compared to the controls. Scale bar = 200 μm.