Table 1.
Primers for reverse transcription-polymerase chain reaction of differentiation-specific genes
| Gene* | Primer sequences (5′–3′) | Position (bp) | Predicted size (bp) |
|---|---|---|---|
| Housekeeping gene | |||
|
GAPDH |
Sense:
GGGCTGCTTTTAACTCTGGT
Antisense:
GCAGGTTTTTCTAGACGG |
134–835 |
702 |
| Adipose tissue-specific genes | |||
|
LPL |
Sense:
GAGATTTCTCTGTATGGCACC
Antisense:
CTGCAAATGAGACACTTTCTC |
1261–1536 |
276 |
|
PPARγ |
Sense:
GCTGTTATGGGTGAAACTCTG
Antisense:
ATAAGGTGGAGATGCAGGCTC |
153–503 |
351 |
| Bone-specific genes | |||
|
ALP |
Sense:
TGGAGCTTCAGAAGCTCAACACCA
Antisense:
ATCTCGTTGTCTGAGTACCAGTCC |
122–575 |
454 |
|
OC |
Sense:
ATGAGAGCCCTCACACTCCTC
Antisense:
GCCGTAGAAGCGCCGATAGGC |
19–312 |
294 |
| Cartilage-specific genes | |||
|
AGN |
Sense:
TGAGGAGGGCTGGAACAAGTACC
Antisense:
GGAGGTGGTAATTGCAGGGAACA |
6591–6910 |
350 |
| COL2A1 | Sense: CAGGTCAAGATGGTC Antisense: TTCAGCACCTGTCTCACCA | 1341–1717 | 377 |
*GAPDH – glyceraldehyde-3-phosphate dehydrogenase; LPL – lipoprotein lipase; PPARγ – peroxisome proliferator-activated receptor-γ; ALP – alkaline phosphatase; OC – osteocalcin; AGN – aggrecan; COL2A1 – collagen type II α1.