Skip to main content
. 2019 Feb 5;11(2):183. doi: 10.3390/cancers11020183

Figure 2.

Figure 2

Targeting HLA-A2.1 restricted Ag to CD169 results in Ag-specific T cell responses in HLA A2.1 transgenic mice. Intravenous immunization with 1 µg Ab:Ag conjugates in the presence of 25 µg anti-CD40 Ab and 25 µg Poly(I:C). (A) Percentage of IFNγ producing CD8+ T cells after 5 h in vitro restimulation with MART-1 peptide (B) Percentage of CD8+ T cells binding HLA-A2.1 tetramers 7 days after immunization with MART-1:Ab conjugates in HLA-A2.1 transgenic mice. Combined data of two experiments with 3–6 mice per group with one representative dotplot of each group is shown. Statistical analysis one-way ANOVA with Sidak’s multiple comparison test * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.