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. 2019 Feb 18;8(2):173. doi: 10.3390/cells8020173

Figure 4.

Figure 4

Effects of human DBMSCs on expression of the cell surface molecules HLADR, CD80, CD86, CD273, and CD274 by human monocytes differentiated into macrophages by GM-CSF, as analyzed by flow cytometry. After six days in culture, compared to untreated macrophages, CMDBMSCs and SFDBMSCs had no significant effects (p > 0.05) on expression of HLA-DR (A), CD80 (B), CD273 (D), and CD274 (E) on macrophages while significantly decreasing expression of CD86 (C). Compared to untreated macrophages, ICDBMSCs had no significant effects (p > 0.05) on expression of HLA-DR (A) and CD80 (B) on macrophages while significantly decreasing expression of CD86 (C), CD273 (D), and CD274 (E) on macrophages. Levels of expression are presented as median fluorescent intensity (MFI) as determined by flow cytometry. Experiments were carried out in duplicate and repeated 30 times using 30 individual preparations of both monocyte-derived macrophages and DBMSCs. * p < 0.05. Bars represent standard errors.