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. 2019 Feb 18;8(2):173. doi: 10.3390/cells8020173

Figure 5.

Figure 5

Effects of human DBMSCs on expression of the intracellular cytokines IL-1β, IL-6, IL-8, IL-12, IFN-γ, and TNF-α by human monocytes differentiated into macrophages by GM-CSF, as analyzed by flow cytometry. After six days in culture, compared to untreated macrophages, CMDBMSCs significantly decreased expression of IL-1β (A) by macrophages while having no significant effects (p > 0.05) on expression of IL-6 (B), IL-8 (C), IL-12 (D), IFN-γ (E), and TNF-α (F) by macrophages. Compared to untreated macrophages, SFDBMSCs significantly decreased expression of IL-1β (A) and IL-12 (D) while having no significant effects (p > 0.05) on expression of IL-6 (B), IL-8 (C), and TNF-α (F), but significantly increasing expression of IFN-γ (E) by macrophages. In addition, compared to untreated macrophages, ICDBMSCs significantly increased expression of IL-1β (A), IFN-γ (E), and TNF-α (F) while decreasing IL-6 (B) and IL-12 (D), but having no significant effects (p > 0.05) on expression of IL-8 (C) by macrophages. Levels of expression are presented as median fluorescent intensity (MFI) as determined by flow cytometry. Experiments were carried out in duplicate and repeated 30 times using 30 individual preparations of both monocyte-derived macrophages and DBMSCs. * p < 0.05. Bars represent standard errors.