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Migration of breast cancer cells cultured on different substrates. MDA-MB-231 and MCF-7 cells were seeded on Millipore filters or Millipore filters covered with Matrigel (0.18 μg/mL) or collagen type I (Coll I) and incubated for 5 h. All assays were performed as described in Materials and Methods. Asterisks (**) indicate statistically significant differences (p ≤ 0.01).
