Figure 2.

HCC1419 and SKBR3 cells were treated with 250 nM lapatinib for 1 and 2 weeks respectively, after which time RNA was isolated from control and treated cells. qRT-PCR was performed for senescence associated genes p15, p21 and p27 and the results are expressed as a fold-change in expression in lapatinib treated cells relative to untreated control cells for each cell line. * p < 0.005; ** p < 0.005 (error bars reflect n = 3, p-values calculated using Students t-test in excel).