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. 2019 Feb 5;11(2):181. doi: 10.3390/cancers11020181

Figure 1.

Figure 1

Cord blood (CB)-derived dendritic cell (DC) (CB-DC) culture containing different myeloid progenitors. (A) Schematic overview of the culture protocol for the generation of the backbone CB-DC. (B) CD11c and HLA-DR expression using flow cytometry after expansion (day 7) and after differentiation (day 14) within the CB culture. (C) Identification of DC progenitors after the expansion phase based on CD34, CD115. CD116, CD117, CD135, CD45RA, CD123, and CD14 expression compared between CD115+, CD34+, and the remaining population (negative). (D) Percentage of the presence of the progenitors in the CB culture. (E) The expression of CD11c and HLA-DR after differentiating the sorted progenitors in differentiation medium. Data represent at least five independent experiments.