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. 2019 Jan 30;8(2):101. doi: 10.3390/cells8020101

Figure 1.

Figure 1

CRF receptors in human plasma, EVs, and human gut. (a) Representative immunoblots showing presence of CRF receptors 1 and 2, B7-2, and CD9 bands in plasma of healthy human subjects and IBS-D patients. Band 5 and 6 of CRFR overlapped with bands in (b) B7-2, and (c) CD9 immunoblots. IBS-D: irritable bowel syndrome patients with diarrhea; HC: healthy controls. (d) Representative confocal micrographs showing localization of CRFR granules in human duodenal and colonic biopsies. Intestinal crypts with CRFR immunoreactivity in goblet cells (arrows) at 40× and 63× magnification are shown. Scale: 50 µm at 40× and 20 µm at 63×. (e) Representative images of negative controls. Primary antibody for CRF-RI/II was excluded, but all other steps were performed in parallel with other sections shown in 1d.