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. 2019 Feb 1;8(2):171. doi: 10.3390/jcm8020171

Figure 1.

Figure 1

(A) miR-122 binding site in the SerpinB3 3’untranslated region (3′UTR) as reported by the TargetScan algorithm. (B) Dual-luciferase assay in HepG2 cells. The SerpinB3 3’UTR-containing vector was co-transfected with miR-122 or negative control (NC). MiR-122 overexpression determined a decrease of the reporter gene activity in pGL3-SerpinB3 co-transfected HepG2 cells. (C) qPCR analysis of miR-122 levels following miR-122 inhibitor (AM-122) or miR-122 mimic transfection in HepG2 cells stably overexpressing SerpinB3 (HepG2/SerpinB3) with respect to controls. U6RNA was used as housekeeping gene. Y-axis reports 2−ΔΔCt levels expressed in logarithmic form. (D,E) qPCR and Western blot analysis of SerpinB3 in transfected HepG2/SerpinB3 cells. β-Actin was used as housekeeping gene. Y-axis reports 2−ΔΔCt levels. NCi: miRNA inhibitor negative control; NC: miRNA precursor negative control. (* p ˂ 0.05). Data shown are representative results of at least three independent experiments.