Skip to main content
. 2019 Feb 1;8(2):171. doi: 10.3390/jcm8020171

Figure 5.

Figure 5

(A–C) In each panel, from the top to the bottom: cell viability and caspase 3/7 activity assays and western blot analysis of cleaved caspase-3 in HepG2 and Hep3B cells overexpressing miR-122 (A,B) and in Huh-7 cells transfected with miR-122 inhibitor (AM-122) (C) following vehicle (NT) or sorafenib administration. β-Actin was used as housekeeping gene in western blot analysis and numbers represent fold-change values. NC: miRNA precursor negative control; NCi: miRNA inhibitor’s negative control. (D) Cell viability assay in SerpinB3-overexpressing HepG2 cells following sorafenib treatment (10 and 50 µM for 48 h). (* p ˂ 0.05 Mann–Whitney test). (E) MiR-122 quantification in SerpinB3-overexpressing HepG2 cells following sorafenib treatment. (* p ˂ 0.05 Mann–Whitney test). (F) Western blot analysis of cleaved Poly(ADP-ribose) polymerase 1 (PARP) and cleaved caspase-3 in HepG2 cells overexpressing SerpinB3 (HepG2/SerpinB3) transfected with miR-122 in untreated or sorafenib (10 µM)-treated cells (48 h). β-Actin was used as housekeeping gene in western blot analysis and numbers represent fold-change values. NC: miRNA precursor negative control.