Domain	Patients Selection	Index Test	Reference Standard	Flow and Timing
Signaling question (yes, no or unclear)	Was a consecutive or random sample of patients enrolled?	Were the index test results interpreted without knowledge of the results of the reference standard?	Is the reference standard likely to correctly classify the target condition?	Was there an appropriate interval between index tests and reference standard?
				Did all patients receive a reference standard?
	Did the study avoid inappropriate exclusions?		Were the reference standard results interpreted without knowledge of the results of the index test?	Did all patients receive the same reference standard?
Risk of bias (high, low or unclear)	Could the selection of patients have introduced bias?	Could the conduct or interpretation of the index test have introduced bias?	Could the reference standard or its interpretation have introduced bias?	Could the study flow have introduced bias?
Concerns about applicability (high, low, or unclear)	Are there concerns that the included patients do not match the review question?	Are there concerns that the index test or its interpretation differ from the review question?	Are there concerns that the target condition as defined by the reference standard does not match the review question?	-