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. 2019 Feb 2;11(2):175. doi: 10.3390/cancers11020175

Figure 1.

Figure 1

Stable transfection of connexin43 (Cx43) in H125 cells (H125-CX43) increases gap junction formation, gap junctional intercellular communication (GJIC), and E-cadherin and β-catenin localization to the plasma membrane (non-transfected parental cells are designated H125 and empty vector-transfected cells are designated H125-NEO). (A) Western blot of Cx43 and densitometric analysis of band densities normalized to tubulin loading control (n = 3 replicate experiments); (B) scrape-loading/dye-transfer assay for GJIC showing Lucifer Yellow-fluorescent dye-loaded cells (top panels) and bright field images (bottom panels), scale bars: 400 µm; (C) quantification of average number of dye-loaded cells perpendicular to the scrape (* p < 0.01, Student’s t-test, mean ± S.D., n = 4 replicate experiments); (D) fluorescent fluorescein isothiocyanate (FITC) immunostaining of Cx43 with 4′,6-diamidino-2-phenylindole (DAPI) staining of nuclei, scale bars: 200 µm.