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. 2019 Feb 2;11(2):175. doi: 10.3390/cancers11020175

Figure 6.

Figure 6

Connexin43 suppresses the expression of stem cell pluripotency markers and tumorsphere formation. (A) Western blots of stem cell pluripotency factors Nanog, Oct4, and Sox2 in H125-NEO and H125-CX43 cells; band densities were normalized to tubulin loading control and to H125-NEO (* p < 0.01 compared to H125-NEO, one-sample t-test, mean ± S.D., n = 3 replicate experiments); (B) images of tumorspheres after 3 weeks culture in stem cell medium; scale bars: 1000 µm (left and right panels) and 400 µm (middle panel); (C) quantification of spheres larger than 50 µm in diameter (mean ± S.D., n = 3 replicate experiments); (D) Western blots of Cx43, Nanog, and CD44 in bulk cells and spheres; band densities were normalized to tubulin loading control and to bulk cells (* p < 0.01 compared to bulk cells, one-sample t-test, mean ± S.D., n = 3 replicate experiments).