Figure 7.

Connexin43 reduces the population of aldehyde dehydrogenase (ALDH)-positive H125 cells. (A) Dot plots of fluorescence assisted cell sorting (FACS)-sorted ALDH-low (R1 pool) and ALDH-high (R2 pool) with and without DEAB in H125-NEO and H125-CX43 cells; the percentages of R2 cells are shown on the plots. (B) Western blots of Cx43 and Nanog in R1 (ALDH-low) and R2 (ALDH-high) populations of H125-NEO and H125-CX43 cells; band densities were normalized to tubulin loading control and to R1 cells (* p < 0.01 compared to R1 cells, one-sample t-test, mean ± S.D., n = 3 replicate experiments).