Figure 4.

Cancer cells formed Homer Wright-like rosettes in bioprintable bioinks as they do in human neuroblastoma tissue. (a) Histological section of human neuroblastoma tumors from two high-risk patients were stained with HE. The tumor masses were examined under light microscope with 10× (first image on the left) and 20× (two images on the right) magnification. Arrows indicate Homer Wright-like rosettes structures. (b,c) Histological sections (first row) and immunocytochemical images (second row) of (b) 0.3% COL and (c) 0.2% COL-0.5% AG after 14 days of in vitro culture show organization of tumor and nontumor cells in non-bioprintable and bioprintable hydrogels, respectively.