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. 2019 Feb 18;11(2):235. doi: 10.3390/cancers11020235

Figure 2.

Figure 2

MYBBP1A knock down increases c-MYB activity. (A) MYBBP1A knock down by the expression of a specific shRNA. Cell lines were transfected with MYBBP1A shRNA (sh) or a scramble vector (V). After selection, cells were grown to 80% confluence, and proteins were extracted. The figure shows the western blot results of MYBBP1A expression in all cell lines. Scale Bar: 8 µM. (B) Quantification of MYBBP1A expression in cells expressing MYBBP1A shRNA (sh) related to the scramble vector (V). (C) MYBBP1A knock down increases the expression of c-MYB target genes. mRNA levels of genes directly transcribed by c-MYB were measured by Q-RT-PCR. Graphs represent mRNA levels of cells expressing MYBBP1A shRNA normalized to mRNA levels of control cells. (D) Percentage of CD34+ cells measured by flow cytometry. (E) Percentage of CXCR4+ cells measured by flow cytometry. (B–D) Graphs show the mean ± SD of three independent experiments performed in triplicate. * p < 0.05; ** p < 0.01.