Figure 2.

Phase 2, evaluation of select antimicrobials against E. coli O157:H7-inoculated beef subprimals in conjunction with blade tenderization. (a) Array of beef subprimals during one trial; (b) beef subprimal prepared for inoculation in labeled zones; (c) inoculated beef subprimal entering blade tenderizer; (d) sprayed and blade-tenderized subprimal being cored; (e) recovery of beef cores using a two-inch circular drill bit; (f) recovered beef cores; (g) sanitizing outside of beef cores by passage through radiant heat oven; (h) sectioning of beef cores; (i) blending and (j) enrichment incubation of beef sections; (k) Bead Retriever^TM for immunomagnetic bead recovery and enrichment of E. coli O157:H7 from enrichment broth; (l) plating of immunomagnetic beads with recovered E. coli O157:H7 onto EMB media containing rifamycin SV.