Table 2.
Quantitative and qualitative parameters along time, reported as mean ± SD, obtained from all dogs from the same group (Treatment and Control group). Different superscripts denote statistically significant differences between columns within groups (lowercase (a and b) to underline statistical differences within T group, uppercase (A and B) within C group); different symbols denote statistically significant differences between times between groups (# in T group vs. § in C group). HOS: hypo-osmotic swelling test.
| Group | Treatment Group | Control Group | ||||||
|---|---|---|---|---|---|---|---|---|
| Parameter | T0 | T30 | T60 | T90 | T0 | T30 | T60 | T90 |
| Sperm-Rich Fraction Volume (mL) | 0.99 ± 0.04 | 1.23 ± 0.39 | 1.03 ± 0.10 | 1.07 ± 0.19 | 0.95 ± 0.08 | 1.24 ± 0.45 | 1.43 ± 0.73 | 1.14 ± 0.35 |
| Concentration (spz × 106/mL) | 390.86 a ± 82.13 | 464.57 a,b ± 153.12 | 873.57 b,# ± 262.04 | 899.43 b,# ± 150.67 | 377.56 ± 72.48 | 420.38 ± 67.8 | 376.79 § ± 62.22 | 387.89 § ± 89.77 |
| Total Sperm Count (spz × 106) | 386.57 a ± 88.22 | 613.71 a,b ± 392.29 | 892.13 b,# ± 247.01 | 956.00 b,# ± 175.85 | 358.83 ± 72.70 | 533.30 ± 177.53 | 567.36 § ± 363.18 | 440.6 § ± 150.8 |
| Total Motility (%) | 93.00 ± 7.44 | 96.43 ± 6.40 | 98.86 ± 1.07 | 98.71 ± 0.76 | 94.43 ± 6.97 | 95.57 ± 6.16 | 95.86 ± 5.28 | 96.29 ± 5.36 |
| Progressive Motility (%) | 51.29 a ± 22.51 | 71.86 a,b ± 9.92 | 77.86 b,# ± 11.51 | 82.86 b,# ± 7.99 | 55.43 ± 14.02 | 65.57 ± 15.49 | 62.14 § ± 12.05 | 68.43 § ± 13.59 |
| Rapid (%) | 73.29 a ± 10.24 | 83.40 a,b,# ± 6.20 | 86.43 b,# ± 3.64 | 86.79 b,# ± 3.58 | 71.43 ± 11.32 | 73.86 § ± 4.79 | 79.71 § ± 6.43 | 80.43 § ± 5.21 |
| Medium (%) | 6.14 a ± 3.34 | 8.86 a,b ± 6.54 | 10.86 a,b,# ± 3.08 | 11.00 b ± 2.31 | 6.43 ± 7.13 | 5.29 ± 3.99 | 5.29 § ± 6.47 | 8.14 ± 3.56 |
| Slow (%) | 11.14 a ± 5.76 | 5.29 a,b,# ± 2.75 | 2.14 b,# ± 1.68 | 1.79 b,# ± 1.73 | 12 A ± 4.69 | 9.86 A,§ ± 3.14 | 6.14 B,§ ± 2.95 | 4.71 B,§ ± 2.05 |
| Static (%) | 9.43 a ± 3.51 | 2.43 b,# ± 1.62 | 0.86 b,# ± 0.38 | 0.71 b,# ± 0.49 | 10.14 A ± 2.1 | 11 A,§ ± 2.56 | 8.86 A,B,§ ± 3.64 | 6.71 B,§ ± 3.41 |
| HOS (% curled) | 91.14 a ± 4.22 | 94.71 a,b,# ± 4.61 | 97.43 b,# ± 1.62 | 96.29 b,# ± 2.36 | 91.14 ± 2.95 | 90.57 § ± 4.27 | 92.14 § ± 5.51 | 92.71 § ± 2.25 |
| Sperm Viability (Eo-Nig) (%) | 91.29 a ± 5.94 | 94.86 a,b,# ± 3.85 | 98.71 b,# ± 1.25 | 98.71 b,# ± 0.95 | 91.13 ± 0.69 | 91.57 § ± 3.64 | 91.86 § ± 3.34 | 93.65 § ± 3.80 |