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Hatching and survival rate of zebrafish embryos. (A) Hatching rate of zebrafish embryos induced by E3 solution, bulk TiO2 and different concentration TiO2 NPs for 0–96 hpf. The results showed no strong inhibition of hatching rate after embryos exposed to TiO2 nanoparticles; (B) Percentage of surviving embryos after 96 h of exposure. Results are presented as mean ± SD from five independent experiments (n = 200, 40 embryos in 5 replicates each).
